Strep throat is a term used to indicate a throat infection caused by bacteria belonging to the genus Streptococcus i.e. group A Streptococcus (GAS), group A beta-hemolytic streptococcus, or Streptococcus pyrogenes.

The infection is not only restricted to the pharynx and larynx but extends upwards to affect tonsils. Being contagious, the disease can spread from person to person by droplet transfer during sneezing or coughing. Although the infection is not restricted to a certain age group, people between 5 to 15 years of age are more prone to contracting the disease.

According to the Centers for Disease Control and Prevention (CDC), the symptoms of a Strep infection include:

1. Throat pain especially while swallowing
2. Inflamed tonsils as well as lymph nodes in the neck region
3. Reddened tonsils with visible white streaks of pus
4. The appearance of petechiae i.e. reddish spots on the upper palate of the mouth 
5. Stomach ache
6. Headache
7. Nausea and vomiting
8. Rash
9. Fever (≥101°F)

Let us understand which tests can be used to make a diagnosis of strep throat.

How to diagnose strep throat?

As soon as a patient refers to a physician, a primary diagnosis of an infection is made based on the symptomatic analysis. In case of prominent strep A symptoms, the healthcare professional asks the patient to take a rapid strep test. These rapid strep tests are of multiple kinds and include the following:

·         Rapid strep antigen test:

Rapid strep antigen test or rapid antigen detection test (RADT) is the first test recommended by physicians to confirm the presence of strep A bacteria. The test works by identifying the antigens of the bacterial cells. To do so, an antibody solution is used which can detect group A Streptococcus within 8 minutes. This way, a rapid strep antigen test can give results within 10 to 20 minutes.

However, when it comes to the accuracy of the results, rapid strep tests are not completely reliable as a false-negative result can be indicated. Therefore, if your physician deems fit, you can be recommended to take an additional throat culture test for confirmation. Furthermore, a positive strep antigen test manifests the presence of a bacterial infection which eliminates the need for any further testing.

·         Throat culture:

Throat culture is performed the same way as a rapid strep test is done. However, in the present case, it may take 24 to 48 hours for the results to beavailable.In case of a positive result, the patient has strep A infection while a negative one indicates that the pathogen involved is other than Streptococcus bacteria.The accuracy of a throat culture is relatively higher than that of a rapid antigen test thus it is considered more reliable.

How to conduct a strep test?

In case of a throat infection, it is important to know the causative agent. If bacteria are responsible for the disease, the most suited treatment is the use of antibiotics.

However, if the infection has a viral basis, there is no need to recommend antibiotic therapy as these drugs do not work against viruses. Therefore, a confirmation regarding the pathogen is needed before starting therapy.

For both kinds of strep tests, the following steps are to be kept in mind while taking a sample:

1. Ask the patient to sit down comfortably and tilt the head backward.
2. Once seated, the patient has to open his or her mouth wide open.
3. At this point, the healthcare professional may use a tongue depressor to increase visualization.
4. A special swab is used to collect the sample which is inserted deeper into the patient’s throat until tonsils are reached.
5. The collected sample is either subjected to a rapid antigen test or sent to the lab for a throat culture.

BD VeritorTM system is a rapid test kit employed for the detection of Strep A streptococcus. This product, along with other different types of test strips, is available on Health Supply 770 at great prices.

Medical equipment used for strep throat test

To conduct a strep throat test, the following testing supplies are needed:

• Tongue blade wooden depressors
• Sterile throat swabs
• Extraction reagent
• Positive and negative control

Comparison of different strep throat tests

According to a study published in the American Family Physician Journal, a comparison was made between the specificity of RADT and throat culture. The findings of the study manifested no significant difference between the two in addition to identical rates of false-negative and false-positive tests.

Moreover, it was revealed that in patients who had recently acquired a strep infection, the reliability of RADT exceeded that of throat culture. Rapid antigen detection tests as well as throat cultures can also be compared based on their working principle, cost, as well as result interpretation, parameters which have been given in the following table:

Conclusion

Pharyngitis, or as it is commonly known, strep throat is a condition caused by Streptococcus bacteria. The infection compromises the patient’s quality of life and needs to be treated. But first, it is essential to evaluate the exact cause of a throat infection which can be done by doing RADT or throat culture. The apparatus needed for both tests is very basic and does not cost much. Therefore, taking a strep test is not only quick and easy but also economical.

Uzma Zafar

PhD Scholar (Pharmaceutics), MPhil (Pharmaceutics), Pharm D, B. Sc.

Uzma Zafar is a dedicated and highly motivated pharmaceutical professional currently pursuing her PhD in Pharmaceutics at the Punjab University College of Pharmacy, University of the Punjab. With a comprehensive academic and research background, Uzma has consistently excelled in her studies, securing first division throughout her educational journey.

Uzma’s passion for the pharmaceutical field is evident from her active engagement during her Doctor of Pharmacy (Pharm.D) program, where she not only mastered industrial techniques and clinical case studies but also delved into marketing strategies and management skills.

Throughout her career, Uzma has actively contributed to the pharmaceutical sciences, with specific research on suspension formulation and Hepatitis C risk factors and side effects. Additionally, Uzma has lent her expertise to review and fact-check articles for the Health Supply 770 blog, ensuring the accuracy and reliability of the information presented.

As she continues her PhD, expected to complete in 2025, Uzma is eager to contribute further to the field by combining her deep knowledge of pharmaceutics with real-world applications to meet global professional standards and challenges.